Faculty of Medical Sciences > Molecular Biology(CFAR) > Research & Publication
Dr. Neetu Singh
Dr. Neetu Singh is presently, working in the area of genomics and transcriptomics of pediatric, breast and lung cancers as well as developmental disorders using different techniques like Microarray, Sequencing and Real-Time PCR. She was also a participant of the Task Force of Halifax Project "Getting to know cancer" to write the reviews on "broad-spectrum integrative design for cancer prevention and therapy" and published series of reviews in "Carcinogenesis" and "Seminars in Cancer Biology". During her Post-Doctoral training from CSIR-Central Drug Research Institute, Lucknow, was actively involved in Cancer Research with projects ranging from the clinical study of association of gene polymorphisms in Breast Cancer, to meta-analysis and investigation of the synergistic therapeutic effect of polyphenols in combination with current drugs in Breast Cancer Cells/Explants. During her Ph.D. program, Also received training in Endocrinology and specifically, worked in the area of Utrerine Glycoprotein regulatory phenomena as a function of treatments with Mucolytics, to gain an insight into the Contraceptive action mechanisms.
With the hands-on-experience with molecular biology tools and techniques vis- -vis gene, transcript and protein levels, She has also been conferred with awards in International Conferences and reviewed manuscripts of high impact factor journals. Also received Women- and Young-Scientist Fellowships from Department of Science and Technology and Travel Grant from CSIR, Government of India. Recently, received and accomplished Affymetrix Tumor Grant Profiling Award, USA (30 samples) for profiling different phases of Chronic Myeloid Leukemia and Indian Council of Medical Research, New Delhi grant for standardization of methods for MSCs Stem Cell Banking through dental pulp and bone marrow. Recently received Terry Fox Foundation Fellowship, Canada on Uveal Melanoma as Co-PI.
Research schemes/projects
Completed Projects
Affymetrix Tumor Grant Profiling Award (40 samples):One among three world-wide-Project Proposal: entitled "Genomics and Transcriptomics of Chronic Myeloid Leukemia in North Indians"-21.05.2014 : To understand the involvement of chromosomal alterations (Genomics) in Chronic Phase, Accelerated Phase and Blast Crisis as well as its correlation with the transcript profile (Transcriptomics) needs further attention. (2014-2015) under PIship of Dr. Neetu Singh, Co-PI Prof. Ravi Kant and Co-PI Dr. A. K.Tripathi.
Indian Council of Medical Research, New Delhi Project for MSCs Stem Cell Banking: Proposal accepted for one year for standardization of methods for "Collection, Isolation and Characterization of Human Dental Pulp Derived Mesenchymal Stem Cells for Banking and Clinical Use: a pilot study" : D.O. No.80/7/2013-BMS dated 15.05.2014under PIship of Prof. Ravi Kant and Co-PIs Dr. Neetu Singh, Dr. Shalini Gupta, Dr. A. K.Tripathi
Currently Running Projects
Ministry of Ayush, Government of India-Effect of Yogic Intervention on QOL & Immunological Profile in Breast Cancer Patients Undergoing Chemotherapy & Radiotherapy- A Randomized Controlled Trial under PIship of Prof. Shailendra Yadav and Co-PI Dr. Neetu Singh.
Terry Fox Foundation Fellowship, Canada on Investigation of Copy Number Variation in Uveal Melanoma tissues by ONCOSCAN FFPE Assay for prognosticating Liver metastasis in patients with Uveal Melanoma under PIship of S.Krishnakumar, Vision Research Foundation, Chennai and Co-PIs (Dr. Neetu Singh, Dr. Sowmya Parameswaran, Dr. Jyotirmay Biswas, Dr. Moutushy Kandalam, Dr. V. Umashankar.
Peer Reviewed List of Publications
Reviews Published:
Goodson WH, 3rd, Lowe L, Carpenter DO, Gilbertson M, Manaf Ali A, Lopez de Cerain Salsamendi A .Singh N et al. Assessing the carcinogenic potential of low-dose exposures to chemical mixtures in the environment: the challenge ahead. Carcinogenesis. 2015;36 Suppl 1:S254-96. IF=5.27; Cited by 1
Ochieng J, Nangami GN, Ogunkua O, Miousse IR, Koturbash I, Odero-Marah V Singh N, et al. The impact of low-dose carcinogens and environmental disruptors on tissue invasion and metastasis. Carcinogenesis. 2015;36 Suppl 1:S128-59. IF=5.27; Cited by 1
Thompson PA, Khatami M, Baglole CJ, Sun J, Harris SA, Moon EY . Singh N, et al. Environmental immune disruptors, inflammation and cancer risk. Carcinogenesis. 2015;36 Suppl 1:S232-53. IF=5.27; Cited by 2
Robey RB, Weisz J, Kuemmerle NB, Salzberg AC, Berg A, Brown DG . Singh N, et al. Metabolic reprogramming and dysregulated metabolism: cause, consequence and/or enabler of environmental carcinogenesis? Carcinogenesis. 2015;36 Suppl 1:S203-31. IF=5.27; Cited by 1
Casey SC, Vaccari M, Al-Mulla F, Al-Temaimi R, Amedei A, Barcellos-Hoff MH . Singh N, et al. The effect of environmental chemicals on the tumor microenvironment. Carcinogenesis. 2015;36 Suppl 1:S160-83. IF=5.27; Cited by 1
Casey SC, Amedei A, Aquilano K, Azmi AS, Benencia F, Bhakta D . Singh N, et al. Cancer prevention and therapy through the modulation of the tumor microenvironment. Seminars in cancer biology. 2015 Dec;35 Suppl:S199-223. doi: 10.1016/j.semcancer.2015.02.007. Epub 2015 Apr 10. IF=9.33 Cited by 6
Kravchenko J, Corsini E, Williams MA, Decker W, Manjili MH, Otsuki T . Singh N, et al. Chemical compounds from anthropogenic environment and immune evasion mechanisms: potential interactions. Carcinogenesis. 2015;36 Suppl 1:S111-27. IF=5.27; Cited by 1
Narayanan KB, Ali M, Barclay BJ, Cheng QS, D'Abronzo L, Dornetshuber-Fleiss R .. Singh N, et al. Disruptive environmental chemicals and cellular mechanisms that confer resistance to cell death. Carcinogenesis. 2015;36 Suppl 1:S89-110. IF=5.27; Cited by 1
Langie SA, Koppen G, Desaulniers D, Al-Mulla F, Al-Temaimi R, Amedei A . Singh N, et al. Causes of genome instability: the effect of low dose chemical exposures in modern society. Carcinogenesis. 2015;36 Suppl 1:S61-88. IF=5.27; Cited by 2
Engstrom W, Darbre P, Eriksson S, Gulliver L, Hultman T, Karamouzis MV .. Singh N, et al. The potential for chemical mixtures from the environment to enable the cancer hallmark of sustained proliferative signalling. Carcinogenesis. 2015;36 Suppl 1:S38-60. IF=5.27; Cited by 1
Carnero A, Blanco-Aparicio C, Kondoh H, Lleonart ME, Martinez-Leal JF, Mondello C . Singh N, et al. Disruptive chemicals, senescence and immortality. Carcinogenesis. 2015;36 Suppl 1:S19-37. IF=5.27; Cited by 1
Nahta R, Al-Mulla F, Al-Temaimi R, Amedei A, Andrade-Vieira R, Bay SN .. Singh N, et al. Mechanisms of environmental chemicals that enable the cancer hallmark of evasion of growth suppression. Carcinogenesis. 2015;36 Suppl 1:S2-18. IF=5.27; Cited by 1
Samadi AK, Bilsland A, Georgakilas AG, Amedei A, Amin A, Bishayee A Singh N, et al. A multi-targeted approach to suppress tumor-promoting inflammation. Seminars in cancer biology. 2015. Dec;35 Suppl:S151-84. doi: 10.1016/j.semcancer.2015.03.006. Epub 2015 May 5. Authors contributed equally arranged in alphabetical order; IF=9.33; Cited by 1
Feitelson MA, Arzumanyan A, Kulathinal RJ, Blain SW, Holcombe RF, Mahajna J Singh N, et al. Sustained proliferation in cancer: Mechanisms and novel therapeutic targets. Seminars in cancer biology. 2015 Dec;35 Suppl:S25-54. doi: 10.1016/j.semcancer.2015.02.006. Epub 2015 Apr 17. IF=9.33; Cited by 2
Hu Z, Brooks SA, Dormoy V, Hsu CW, Hsu HY, Lin LT . Singh N, et al. Assessing the carcinogenic potential of low-dose exposures to chemical mixtures in the environment: focus on the cancer hallmark of tumor angiogenesis. Carcinogenesis. 2015;36 Suppl 1:S184-202. IF=5.27; Cited by 1
Block KI, Gyllenhaal C, Lowe L, Amedei A, Amin AR, Amin A, Le A, .................. Singh N et al. Designing a broad-spectrum integrative approach for cancer prevention and treatment. Semin Cancer Biol. 2015 Dec;35 Suppl:S276-304. doi: 10.1016/j.semcancer.2015.09.007. IF=9.33; Cited by 2
Manuscripts Published
Singh N, Sahu DK, Chowdhry R, Mishra A, Agarwal P, Goel MM, Chandra A, Singh SK, Srivastava C, Ojha BK, Gupta DK!, Kant R!. IsoSeq analysis and functional annotation of the infratentorial ependymoma tumor tissue on PacBio RSII platform. Meta Gene. 2016 Feb;7: 70 75. (Most Downloaded Meta Gene Articles).
Singh N, Sahu DK, Mishra A, Agarwal P, Goel MM, Chandra A, Singh SK, Srivastava C, Ojha BK!, Gupta DK!, Kant R!. Multiomics approach showing genome-wide copy number alterations and differential gene expression in different types of North-Indian pediatric brain tumors. Gene. 2016 Feb 1;576(2 Pt 2):734-42. (5-Yr impact factor: 2.185) cited by 1
Nigam M, Singh N, Ranjan V, Zaidi D, Sharma R, Nigam D, et al. Centchroman mediated apoptosis involves cross-talk between extrinsic/intrinsic pathways and oxidative regulation. Life sciences. 2010;87(23-26):750-8. Impact Factor 2.701 Cited by 11; SIngh N as first author
Singh N, Malaviya B. Kinetics of bromhexine-mediated down-regulation of focal adhesive molecules of uterus and trophectoderm affecting conception in the rat. Contraception. 2006;73(6):645-53. Impact Factor 2.847; Cited by 2
Meta-analysis:
Pabalan N, Singh N, Pineda MR, Jarjanazi H. Meta-analysis of the association between PTPN11 G/A polymorphism at intron 3 with risk of gastric atrophy among East Asians. Journal of gastrointestinal cancer. 2014;45(3):319-24. IF=0.38; cited by 1
Case Report:
Singh N, Sahu DK, Purwar P, Gupta SK, Tripathi AK, Dixit J, Kant R, Gupta DK. CGH Array based analysis of a patient suffering with Amelogenesis Imperfecta, Jalili Syndrome, Situs Inversus and oligozoospermia. J genetic disorders and genetic reports. 2015;4:1. Authors contributed equally
Singh N, Sharma R, Verma UP, Singh SK, Dixit J, Balapure AK. Rosai-Dorfman disease in an Asian Indian woman with classic generalized lymphadenopathy and nasal obstruction: a case report. Ear, nose, & throat journal. 2009;88(3):E20-3. IF=0.88; cited by 1
Abstract:
Singh N, Tripathi A K, Sahu DK, Mishra A, Chowdhry R, Kant R. Combination of multiomics approach and BCR-ABL based molecular-testing: A better way to monitor treatment response, resistance and disease-progression in Chronic Myeloid Leukemia. European J Cancer. 2016;54 (Suppl I): S1-S72. Impact Factor 5.41
Singh N, Sahu DK, Ojha BK, Srivastava C, Gupta DK. Analysis of chromosomal abberations in North-Indian Medulloblastoma. 4th World Congress on Cancer Science and Therapy. October 20-22, 2014 Scientific Tracks Abstracts: J Cancer Sci Ther, DOI: 10.4172/1948-5956.S1.033 Impact Factor 3.09
Nigam M, Ranjan V, Singh N, Srivastava S, Sharma R, Balapure AK. Involvement of p53, p21 and Bcl2 Family members in Centchroman induced apoptosis in MCF-7 and MDA MB-231 Human Breast Cancer Cells". Poster & Abstract at: 12th International Conference on the Interface of Chemistry-Biology in Biomedical Research (ISCBC-2008), at Birla Institute of Technology and Science, Pilani-333031 (Rajasthan), IndiaFeb 20th-24th, 2008.
Singh SVS, Singh N, Garg VK, Chaturvedi R, Sharma M, Rath SK. Single Nucleotide Polymorphism in p21 p53 and cox2 gene with the risk of Breast Cancer in North Indian sub populations. A case control study. Poster and Abstract at 14th Annual National Conference of Breast Cancer Foundation of India held at CSMMU (BCF-CON 2010). BEST POSTER PRIZE
Singh SVS, Mitra AK, Singh N, Garg VK, Chaturvedi R, Sharma M, Rath SK. Gene polymorphism in Squamous Cell Carcinoma of Head, Neck and Breast Cancer: our experience.Symposium on "DNA repair, Genomic Instability and Cancer".
Dr. Ashutosh Shrivastava
Dr. Ashutosh Shrivasava obtained his Doctorate from Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow in 2007. Subsequently he pursued Postdoctoral fellowship from Harvard Medical School, Boston, USA. He joined the Department of Molecular Biology, in The Center for Advance Research as an Assistant Professor in early 2016. Dr. Shrivastava aim to identify Metabolomics based markers for early detection of Cancer and to find novel therapeutic targets for anticancer drug development. Additionally Dr. Shrivastava plan to contribute to the hospital functioning by introducing molecular and metabolism based markers for diagnostics of inborn errors of metabolism.
Tiwari M, Kumar A, Sinha RA, Shrivastava A, Balapure AK, Sharma R, Bajpai VK, Mitra K, Babu S, Godbole MM. Mechanism of 4-HPR-induced apoptosis in glioma cells: evidences suggesting role of mitochondrial-mediated pathway and endoplasmic reticulum stress. Carcinogenesis. 2006;27(10):2047-58.
Shrivastava A, Tiwari M, Sinha RA, Kumar A, Balapure AK, Bajpai VK, Sharma R, Mitra K, Tandon A, Godbole MM. Molecular iodine induces caspase-independent apoptosis in human breast carcinoma cells involving the mitochondria-mediated pathway. J Biol Chem. 2006;281(28):19762-71.
Kumar A, Sinha RA, Tiwari M, Pal L, Shrivastava A, Singh R, Kumar K, Gupta SK, Godbole MM. Increased pro-nerve growth factor and p75 neurotrophin receptor levels in developing hypothyroid rat cerebral cortex are associated with enhanced apoptosis. Endocrinology. 2006;147(10):4893-903.
Kumar A, Sinha RA, Tiwari M, Singh R, Koji T, Manhas N, Rastogi L, Pal L, Shrivastava A, Sahu RP, Godbole MM. Hyperthyroidism induces apoptosis in rat liver through activation of death receptor-mediated pathways. J Hepatol. 2007;46(5):888-98.
Sinha RA, Pathak A, Kumar A, Tiwari M, Shrivastava A, Godbole MM. Enhanced neuronal loss under perinatal hypothyroidism involves impaired neurotrophic signaling and increased proteolysis of p75(NTR). Mol Cell Neurosci. 2009;40(3):354-64.
Yadav S, Gupta SK, Godbole MM, Jain M, Singh U, V Pavithran P, Boddula R, Mishra A, Shrivastava A, Tandon A, Ora M, Chowhan A, Shukla M, Yadav N, Babu S, Dubey M, Awasthi PK. Persistence of severe iodine-deficiency disorders despite universal salt iodization in an iodine-deficient area in northern India. Public Health Nutr. 2010;13(3):424-9.
Rai R, Shrivastava A, Tandon A, Godbole MM, Kumar S, Das V, Dwivedi V, Pal L. Human sodium iodide symporter (hNIS) in fibroadenoma breast a immunohistochemical study. Indian J Exp Biol. 2011;49(2):113-7.
Shrivastava A, Kuzontkoski PM, Groopman JE, Prasad A. Cannabidiol induces programmed cell death in breast cancer cells by coordinating the cross-talk between apoptosis and autophagy. Mol Cancer Ther. 2011;10(7):1161-72.
Tandon A, Shrivastava A, Kumar A, Prayaga AK, Sundaram C, Godbole MM. Sodium Iodide Symporter, Estrogen Receptor and Progesterone Receptor Expression in Carcinoma Breast - an Immunohistochemical Analysis. Indian J Pathol Microbiol. 2011;54(4):745-51.
Zhu B1, Shrivastava A1, Luongo C, Chen T, Harney JW, Marsili A, Tran TV, Bhadohouria A, Mopala R, Steen A,Larsen PR, Zavacki AM. Catalysis leads to post-translational inactivation of the Type-1 deiodinase and alters its conformation. J Endocrinol. 2012; 214(1):87-94.(1 Equal first author).
Prasad A, Kuzontkoski PM, Shrivastava A, Zhu W, Li DY, Groopman JE. Slit2N/Robo1 Inhibit HIV-gp120-Induced Migration and Podosome Formation in Immature Dendritic Cells by Sequestering LSP1 and WASp. PLoS One. 2012; 7(10):e48854.
Prasad A, Shrivastava A, Papadopoulos E, Kuzontkoski PM, Reddy MV, Gillum AM, Kumar R, Reddy EP, Groopman JE. Combined administration of rituximab and ON013105 induces apoptosis in mantle cell lymphoma cells and reduces tumor burdenin a mouse model of mantle cell lymphoma. Clin Cancer Res. 2013;19(1):85-95.
Shrivastava A2. The Mechanisms of Mitochondria-mediated Apoptosis in Cancer Chemotherapy. Cell Biology.2015; 3(2-1): 17-21. (2 Corresponding Author)
Shrivastava A, Prasad A, Kuzontkoski PM, Yu J, and Groopman JE. Slit2N Inhibits Transmission of HIV-1 from Dendritic Cells to T-cells by Modulating Novel Cytoskeletal Elements. Sci Rep. 2015; 5:16833.